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GC Content Calculator

Find the GC content and base composition of a nucleotide sequence.

How to use

  1. Enter your values in the fields above.
  2. Press Calculate to see your result instantly.
  3. Use the Share button to copy a link to your result.

About this calculator

This calculator finds the GC content of a DNA or RNA sequence — the percentage of bases that are guanine (G) or cytosine (C) rather than adenine (A) and thymine/uracil (T/U): GC% = (G+C) / total bases × 100. Because G pairs with C via three hydrogen bonds (versus two for A-T), higher GC content generally means a more thermally stable double helix with a higher melting temperature.

Molecular biologists use GC content to design PCR primers, estimate the annealing temperature for lab protocols, assess genome composition across species, and flag potential sequencing or assembly issues, since extreme GC content (very high or very low) is harder to sequence accurately. Paste a DNA or RNA sequence to get its GC percentage and full base composition breakdown.

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